Transmittance between an amylase and starch solution Essay Example For Students

Transmittance between an amylase and starch solution Essay Table 1: Color and Percent Transmittance between an amylase and starch solution with a pH of 6.0 tested in 3 minute intervals Time Color Percent ( % ) Transmittance Average Rate per minute 3 Dark Navy 6 Dark Navy 9 Dark Navy 12 Brown 0.2 0.0667 15 Dark Orange 8.2 2.6667 18 Orange 28.3 6.7 21 Orange 62.3 11.3333 24 Orange 67.4 1.7 27 Bright Orange 79.1 3.9 30 Bright Orange 85.3 2.0667 33 Bright Orange 87.7 0.8333 36 Bright Orange 90.2 -0.6333 39 Bright Orange 88.3 -0.6333 42 Bright Orange 83 -1.7667 45 Orange 74.4 -2.8667 48 Bright Orange 88.4 4.6667 51 Bright Orange 100 3.8667 Average rate=percent transmission at t 2 per centum transmission at t1 / T 2 T 1 Figure 1. Sum of visible radiation transmitted through a pH of 6.0 over clip in 3 minute intervals Table 2: Color and Percent Transmittance between an amylase and starch solution with a pH of 7.0 tested in 3 minute intervals Table 2 Time Color Percent ( % ) Transmittance Average Rate per minute 3 Dark Navy 6 Dark Navy 9 Blue/Brown 0.1 0.0333 12 Dark Orange 3.5 1.1333 15 Orange 21.3 5.9333 18 Light Orange 67.1 15.2667 21 Light Orange 58.8 -2.7667 24 Light Orange 72.3 4.5 27 Bright Orange 76.3 1.3333 30 Bright Orange 81.1 1.6 33 Bright Orange 77.6 -1.1667 36 Bright Orange 85.5 2.6333 39 Bright Orange 73.4 -4.0333 42 Bright Orange 91.2 5.9333 45 Bright Orange 73.8 -5.8 48 Bright Orange 86.8 4.3333 51 Bright Orange 95.4 2.8667 54 Bright Orange 97.6 0.7333 57 Bright Orange 98.9 0.4333 60 Bright Orange 98.8 -0.0333 63 Bright Orange 91.8 -2.333 66 Bright Orange 100 2.7333 Average rate=percent transmission at t 2 per centum transmission at t1 / T 2 T 1 Figure 2. Sum of visible radiation transmitted through a pH of 7.0 over clip in 3 minute intervals Table 3: Color and Percent Transmittance between an amylase and starch solution with a pH of 7.0 tested in 3 minute intervals Table 3 Time Color Percent ( % ) Transmittance Average Rate per minute 3 Dark Navy 6 Dark Navy 9 Blue/Brown 0.2 0.667 12 Dark Orange 10.1 3.3 15 Orange 33.8 7.9 18 Light Orange 48.6 4.9333 21 Light Orange 56.4 2.6 24 Light Orange 77.6 7.0667 27 Bright Orange 78.2 0.2 30 Bright Orange 83.7 1.8333 33 Bright Orange 80.1 -1.2 36 Bright Orange 88.5 2.8 39 Bright Orange 67.3 -7.0667 42 Bright Orange 82.2 4.9667 45 Bright Orange 78.2 -1.3333 48 Bright Orange 85.5 2.4333 51 Bright Orange 94.8 3.1 54 Bright Orange 82.4 -4.1333 57 Bright Orange 100 5.8667 Average rate=percent transmission at t 2 per centum transmission at t1 / T 2 T 1 Figure 3. Sum of visible radiation transmitted through a pH of 7.0 over clip in 3 minute intervals Table 4: Color and Percent Transmittance between an amylase and starch solution with a pH of 8.0 tested in 3 minute intervals Table 4 Time Color Percent ( % ) Transmittance Average Rate per minute 3 Dark Navy 6 Dark Navy 9 Dark Brown 0.1 0.0333 12 Dark Brown 0.2 0.0333 15 Dark Orange 4.9 1.5667 18 Orange 19.4 4.8333 21 Orange 34.4 5 24 Orange 27.4 -2.3333 27 Orange 48.9 7.1667 30 Light Orange 58.1 3.0667 33 Light Orange 62.3 1.4 36 Light Orange 72.9 3.5333 39 Orange 64.6 -2.7667 42 Orange 78.7 4.7 45 Orange 73.5 -1.7333 48 Orange 74.1 0.2 51 Light Orange 80.8 2.2333 54 Bright Orange 87.3 2.1667 57 Bright Orange 88.5 0.4 60 Bright Orange 87.1 -0.4667 63 Bright Orange 87.8 0.2333 66 Bright Orange 90 0.7333 69 Bright Orange 90.5 0.1667 72 Bright Orange 82.4 -2.7 75 Bright Orange 78.9 -1.1667 78 Bright Orange 76.4 -0.8333 81 Bright Orange 93.1 5.5667 84 Bright Orange 94 0.3 .u8baeeca3a8f75cff6333ede8064fddc3 , .u8baeeca3a8f75cff6333ede8064fddc3 .postImageUrl , .u8baeeca3a8f75cff6333ede8064fddc3 .centered-text-area { min-height: 80px; position: relative; } .u8baeeca3a8f75cff6333ede8064fddc3 , .u8baeeca3a8f75cff6333ede8064fddc3:hover , .u8baeeca3a8f75cff6333ede8064fddc3:visited , .u8baeeca3a8f75cff6333ede8064fddc3:active { border:0!important; } .u8baeeca3a8f75cff6333ede8064fddc3 .clearfix:after { content: ""; display: table; clear: both; } .u8baeeca3a8f75cff6333ede8064fddc3 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u8baeeca3a8f75cff6333ede8064fddc3:active , .u8baeeca3a8f75cff6333ede8064fddc3:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u8baeeca3a8f75cff6333ede8064fddc3 .centered-text-area { width: 100%; position: relative ; } .u8baeeca3a8f75cff6333ede8064fddc3 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u8baeeca3a8f75cff6333ede8064fddc3 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u8baeeca3a8f75cff6333ede8064fddc3 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u8baeeca3a8f75cff6333ede8064fddc3:hover .ctaButton { background-color: #34495E!important; } .u8baeeca3a8f75cff6333ede8064fddc3 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u8baeeca3a8f75cff6333ede8064fddc3 .u8baeeca3a8f75cff6333ede8064fddc3-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u8baeeca3a8f75cff6333ede8064fddc3:after { content: ""; display: block; clear: both; } READ: Anne Frank Essay87 Bright Orange 94.1 0.0333 90 Bright Orange 100 1.9667 Average rate=percent transmission at t 2 per centum transmission at t1 / T 2 T 1 Figure 4. Sum of visible radiation transmitted through a pH of 8.0 over clip in 3 minute intervals Table 5: Color and Percent Transmittance between an amylase and starch solution with a pH of 8.0 tested in 3 minute intervals Table 5 Time Color Percent ( % ) Transmittance Average rate per minute 3 Dark Navy 6 Dark Navy 9 Dark Navy 12 Dark Navy 15 Dark Navy 18 Dark Navy 21 Dark Navy 24 Dark Navy 0.1 0.0333 27 Dark Brown 0.1 30 Dark Orange/Brown 0.1 33 Orange/Brown 0.4 0.1 36 Dark Orange 1.4 0.3333 39 Dark Orange 3.5 0.7 42 Dark Orange 11.1 2.5333 45 Dark Orange 10.5 -0.2 48 Dark Orange 16.2 1.9 51 Dark Orange 21.3 1.7 54 Dark Orange 26.4 1.7 57 Dark Orange 32.7 2.1 60 Dark Orange 33.5 0.2667 63 Orange 45.7 4.0667 66 Orange 46.3 0.2 69 Orange 42.1 -1.4 72 Orange 64.8 7.5667 75 Orange 52.5 -4.1 78 Orange 54.3 0.6 81 Orange 54.6 0.1 84 Orange 53.8 -0.2667 87 Orange 50 -1.2667 90 Orange 55.5 1.8333 93 Light Orange 65.7 3.4 96 Light Orange 62 -1.2333 Average rate=percent transmission at t 2 per centum transmission at t1 / T 2 T 1 Figure 5. Sum of visible radiation transmitted through a pH of 9.0 over clip in 3 minute intervals Table 6. Entire Rate transmission per minute of different pH groups. Changing pH Rate transmission per minute pH 6.0 1.961 ( % transmittance/min ) pH 7.0 1.515 ( % transmittance/min ) pH 7.0 ( control ) 1.754 ( % transmittance/min ) pH 8 1.111 ( % transmittance/min ) pH 9 0.646 ( % transmittance/min ) Entire Rate = entire transmission / stoping clip Figure 6. Percent Transmittance of an amylase amylum solution in 3 minute intervals with changing pH Starch Hydrolysis Discussion This experiment was conducted to demo if changing the pH in an environment will alter the rate of the reaction between amylum and amylase. The pH of the amylum amylase solution was the independent variable, and the sum of light being transmitted through the solution after adding I was the dependent variable. The tabular arraies and figures determine the reaction rate by plotting the per centum of visible radiation transmitted versus clip measured to acquire an equation. The void hypothesis of changing the pH of the enzyme solution will non impact the reaction between amylum and amylase is in wrong harmonizing to tabular arraies 1 -5. The changing pH groups in a solution had different rates harmonizing to table 6. The consequences showed that the first pH to make 100 % was the pH of 6.0. Harmonizing to table 1 it took 51 proceedingss for the pH 6.0 solution to make 100 % transmission. The solution of pH 7.0 took 57 proceedingss to make 100 % transmission harmonizing to table 3. Merely 6 proceedingss from the pH of 6.0. Harmonizing to table 2 the 2nd pH of 7.0 ( control ) showed 100 % transmission at 66 proceedingss. The solution of pH 8.0 took 90 proceedingss to make 100 % transmission harmonizing to table 4, most probably because it is more basic. The pH of 9.0 was either a dark naval forces or dark orange for most of the experiment. The solution of pH of 9.0 did non make 100 % transmission. Harmonizing to table 5 the pH of 9.0 solution took a sum of 96 proceedingss to make a transmission of merely 62 % .The pH of 9.0 solution ran out before it reached 100 % . Refering to the informations in figure 5 it took about 40 proceedingss earlier much visible radiation was transmitted for pH of 9.0. The solution of pH 9.0 had the slowest rate of the 5 changing pH solutions ; the rate was 0.646 harmonizing to table 6. The pH with the fastest rate was pH 6.0 with a rate of 1.961. .u2eac118125bd25032741858a4a5c689f , .u2eac118125bd25032741858a4a5c689f .postImageUrl , .u2eac118125bd25032741858a4a5c689f .centered-text-area { min-height: 80px; position: relative; } .u2eac118125bd25032741858a4a5c689f , .u2eac118125bd25032741858a4a5c689f:hover , .u2eac118125bd25032741858a4a5c689f:visited , .u2eac118125bd25032741858a4a5c689f:active { border:0!important; } .u2eac118125bd25032741858a4a5c689f .clearfix:after { content: ""; display: table; clear: both; } .u2eac118125bd25032741858a4a5c689f { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u2eac118125bd25032741858a4a5c689f:active , .u2eac118125bd25032741858a4a5c689f:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u2eac118125bd25032741858a4a5c689f .centered-text-area { width: 100%; position: relative ; } .u2eac118125bd25032741858a4a5c689f .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u2eac118125bd25032741858a4a5c689f .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u2eac118125bd25032741858a4a5c689f .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u2eac118125bd25032741858a4a5c689f:hover .ctaButton { background-color: #34495E!important; } .u2eac118125bd25032741858a4a5c689f .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u2eac118125bd25032741858a4a5c689f .u2eac118125bd25032741858a4a5c689f-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u2eac118125bd25032741858a4a5c689f:after { content: ""; display: block; clear: both; } READ: Vigee Le Brun EssayMentioning the information from figure 6 the pH 0f 9.0 had the lowest per centum transmission. Not excessively far behind was the pH of 8.0. The solutions with a pH of 6.0, 7.0 and the control group were near in per centum transmission. Decision In decision the hypothesis for the amylum, amylase experiment was right. Yes changing the pH of the enzyme solution will impact the rate of the reaction between amylum and amylase. The 5 different pH groups, pH 6.0,7.0, 7.0 ( control ) 8.0 and 9.0 used in this experiment all had different reaction rates and different times when the per centum transmission reached 100 % .It was discovered that the information dropped a few times. This could hold been because the standardization was non completed plenty. This besides could hold been because cuvettes may hold had finger Markss and non decently wiped off. Another inquiry to be proposed would be, does changing the pH in different solutions change the reaction rate between amylum and amylase?